If only RPM is given, RCF can be calculated using this formula:RCF = 0.00001118 x r x RPM 2 Therefore, RCF should be used to describe the centrifuge speed. RCF is dependent on the length of the radius of the rotor, thus the same RPM may not generate the same g force in another centrifuge. Processing CSF specimensA note about centrifugation: g (1 x gravity) represents relative centrifugal force (RCF), but the recommended centrifugation speed is often listed in protocols as revolutions per minute (RPM).
FLUID FOR MAC INSTRUCTIONS MANUAL
Microbiologists should refer to clinical microbiology manuals, such as the American Society for Microbiology’s Manual of Clinical Microbiology, for procedures to identify other bacteria. influenzae from clinical specimens collected from suspected cases of bacterial meningitis, the methods described here will not allow for identification of other isolates that may be of clinical importance but are less likely encountered. influenzae are not as extensively reported, fatal infections with these bacteria can occur, and vaccination against these organisms may be recommended in some laboratories.īecause the primary purpose of this manual is to aid in the identification of N. While laboratory-acquired infections with S.
Additional health and safety information can be found in Chapter 4: Biosafety. meningitidis should strongly consider vaccination. Personnel who are at risk of routine exposure to aerosolized N. pneumoniae, and 9: Identification and Characterization of H. meningitidis, 8: Identification and Characterization of S.
influenzae are presented in the next chapters of this laboratory manual ( Chapters 7: Identification and Characterization of N. Methods for confirmatory identification of N. influenzae can be made on the basis of a cytological examination of the CSF, specific colony morphology on blood and/or chocolate agar, staining properties on a Gram stain, or by detection of specific antigens in the CSF by a latex agglutination test or using a rapid diagnostic test (RDT).
Laboratories may also receive joint fluid, pleural fluid, or other sterile site specimens from these patients. Microbiology laboratories commonly receive cerebrospinal fluid (CSF) or blood specimens from patients with meningitis, pneumonia, or unexplained febrile illness.
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